首页> 外国专利> METHOD FOR OBTAINING FERTILIZED SEXUAL PRODUCTS FROM SEXUAL ADULT FISH.

METHOD FOR OBTAINING FERTILIZED SEXUAL PRODUCTS FROM SEXUAL ADULT FISH.

机译:从性成鱼中获得受精性产品的方法。

摘要

The invention relates to a process for obtaining ready-for-fertilization sexual products from sexually mature fish in a period independent of their natural spawning season, in which actual stage of ripening is determined by probing of sexual products [sperm or eggs], fish not yet ripe for ovulation are separated from ripe fish, compounds of hormonal effect are administered to fish, and after the ovulation sexual products are obtained from the fish or from their environment. In the process according to the invention a nonapeptide-C1-4-alkyl-amide or a decapeptide amide of the general formula (I), Glp-His-Trp-Ser-Tyr-X1-X2-X3-Pro-X4 (I) wherein X1 is a glycyl group or a D-isomer of any natural or synthetic amino acid group, X2 represents an L-amino acid group having 1 to 4 carbon atoms in the side chain, L-phenyl-alanyl or L-tryptophyl group, X3 represents an L-amino acid group having a C1-4 alkyl or C2-4 alkyl-amide side chain, X4 is a glycine-amide or a C1-4 alkyl-amide group, as well as salts or metal-complexes of these compounds are administered to fish in a dose of 0.1 mu g to 5 mg, and to fish not yet ripe for ovulation the above quantities are administered in at least 2 but at most 12 portions, wherein the last dose used is at least the same or advantageously at least 1.5 times higher than the previous one. The process according to the invention makes possible the obtaining of ripe sexual products ready for natural or artificial fertilization from any fish species in any period, independently of the natural spawning season of fish.
机译:本发明涉及一种在独立于其自然产卵季节的时期内从有性成熟的鱼中获得易于受精的有性产品的方法,其中,实际的成熟阶段是通过探测有性产品[精子或卵]来确定的,将成熟的排卵与成熟的鱼分开,将具有荷尔蒙作用的化合物施用于鱼,并在排卵后从鱼或其环境中获得性产品。在根据本发明的方法中,通式(I)的九肽-C1-4-烷基酰胺或十肽酰胺,Glp-His-Trp-Ser-Tyr-X1-X2-X3-Pro-X4(I ),其中X1是糖基或任何天然或合成氨基酸基团的D-异构体,X2代表在侧链上具有1-4个碳原子的L-氨基酸基团,L-苯基-丙氨酰基或L-色氨酸基团,X 3表示具有C 1-4烷基或C 2-4烷基-酰胺侧链的L-氨基酸基团,X 4是甘氨酸-酰胺或C 1-4烷基-酰胺基团,以及它们的盐或金属配合物。这些化合物以0.1微克至5毫克的剂量施用于鱼类,未排卵的鱼类则以至少2但最多12份的形式施予上述量,其中最后使用的剂量至少相同或比上一个高至少1.5倍。根据本发明的方法使得可以在任何时期从任何鱼类中获得准备用于自然或人工受精的成熟有性产品,而与鱼类的自然产卵季节无关。

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