PROCESS FOR OBTAINING A SOLUBLE TRIIODO-THYRONINE ENZYMATIC COMPLEX SUITABLE FOR IMMUNOLOGICAL DETERMINATION OF TRIIODO THYRONINE

摘要

A composition for diagnostic use is disclosed, more particularly for the dosage of tri-iodo thyronine in human blood serum: the subject composition comprises tri-iode thyronine as such and an enzyme which is covalently bonded to tri-iodo thyronine. A diagnostic method is also disclosed, which is intended for using the above specified composition in diagnostic runs. The method, briefly resumed, consists removing the tri-iodo thyronine of the serum sample being assayed by means of an insolubilized anti-T3 antibody and treating the residue with the sensibilized enzyme which is nothing else than the covalent formed by tri-iodo thyronine and an appropriate enzyme, such as carbonic anhydrase.