Liquid phase chromatographic protein sepn. or purificn. - esp. of influenza virus using silica gel passivated with a hydroxylic polymer and then with another protein

摘要

A method is given for the liquid phase chromatographic sepn. of a protein (I) with a solid support (II) passivated with an aqs. soln. of a non-proteinaceous polymer (III). The novelty is that (II) is subjected to a second passivating process prior to chromatography by contacting it with an aqs. soln. of a protein (IV) whose mol. wt. is (I) and which is adsorbable on (II). Appts. is also claimed. (II) is silica gel or alkali metal silicates in granular form (40-200 mu m) having an ave. pore size of 5-200 mu m, and a specific surface of 500-20 m.2/g. (III) pref. contains OH gps. and may be polyethylene glycol, polypropylene glycol, PVA, polybutylene glycol, PVP, poly(ethylenepropylene) glycol etc. and has a pref. mol. wt. of 5000-30000. The process is esp. suitable for the sepn. and purification of viruses, esp. influenza viruses, from their culture media. Almost total recovery of (I) is attainable.