METHOD FOR PRODUCING AND MAINTAINING LEUKOTAXIN PREPARATES CONTAINING ANAPHYLATOXIN AND COCYTOTAXIN AND ANAPHYLATOXIN AND COCYTOTAXIN PROTEINS IN A MOLECULAR HOMOGENIC AND BIOLOGICALLY ACTIVE FORM.

摘要

The invention relates to a process for producing and obtaining anaphylatoxin- and cocytotaxin-containing leucotaxine preparations and anaphylatoxin and cocytotaxin proteins in molecularly homogeneous, biologically active form from contact-activated mammalian serum by the following steps: separation of the proteins from other serum constituents to obtain a serum protein concentrate fraction, separation of a part of accompanying foreign blood proteins from anaphylatoxin and cocytotaxin present in the said protein concentrate fraction, isolation of the leucotaxine preparation by chromatography on hydroxyapatite, and optionally further purification and/or separation of the leucotaxine preparation into anaphylatoxin and cocytotaxin proteins by chromatographical methods. The invention is characterized in that, prior to chromatography on hydroxyapatite, in the above-mentioned process, a major fraction of accompanying foreign blood constituents is separated from the said serum protein concentrate fraction by fractional elution and/or precipitation with a water-soluble alcohol and/or at least one molecular sieve filtration.