Method and reagent for the determination of glycerol using a competitive inhibitor for glycerol kinase

摘要

To determine glycerol in free or bound form, this is reacted with ATP in the presence of glycerol kinase (GK) and optionally a hydrolase to form glycerol-3-phosphate and ADP and determination of one of these reaction products with the aid of at least one downstream enzymatic reaction, where the determination is carried out kinetically and for this purpose the reaction with ATP for the overall reaction is determined to determine the rate and is allowed to proceed according to the pseudo-first order by adding a sugar of the general formula I IMAGE in which the C atoms 2 and 3 D-threo- Have configuration and R represents a carbohydrate residue with 1 to 3 carbon atoms, in which an OH group can also be replaced by a hydrogen atom.