Metachromatic dye sorption and fluorescent light emmisive means for differential determination of developmental stages of neutrophilic granulocytic cells and other leukocytes

摘要

Differentiation, identification and enumeration of dye responsive human blood cells essential to diagnosis and prognosis of certain diseases is established by preparation of the reaction product of a human blood specimen of the patient in an aqueous fixative-free environment at blood temperatures with selected basic, cationic quaternary dyes, particularly basic orange #21. The so-prepared specimen becomes instantly responsive to emissive wave energy source exposure to stimulate reproducible fluorescent light emission from all of said dye responsive cells. The quantum of said fluorescent light energy emitted provides individual sets of data response relative to hue, value, chroma and intensity to permit differentiation, identification and enumeration of all of said dye responsive components in said blood specimen. By use of a bi-modal wave energy system involving additional use of white light wave energy absorbance, independent sets of data can provide both confirmatory and ancillary information.