Tumor necrosis factor and derivatives and mutant forms thereof, the feet. for manufacturing equi. thereof, the feet. for the isolation thereof from a mixture with other proteins, compositions thereof, DNA coding therefor, the DNA is incorporated into a replicable vector and the cell transformed therewith

摘要

Methods and compositions are provided for the purification of a protein identified as tumor necrosis factor from nonrecombinant and recombinant sources, the preparation of nucleic acid encoding this tumor necrosis factor and for its expression in recombinant hosts. This tumor necrosis factor is used in the therapeutic treatment of malignant tumors, either alone or in synergistic combination with an interferon.