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FLUORESCENCE POLARIZATION DETERMINATION, REAGENTS AND METHOD FOR PREPARING REAGENTS FOR DIGITOXIN DETERMINATION
FLUORESCENCE POLARIZATION DETERMINATION, REAGENTS AND METHOD FOR PREPARING REAGENTS FOR DIGITOXIN DETERMINATION
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机译:荧光极化测定,试剂和制备地高辛的试剂
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摘要
The present invention is directed to an improved fluorescent polarization assay for digitoxin, and to improved reagents for use in the assay. The reagent is formed as the reaction product of 3-amino- 3-deoxydigitoxigenin and 5- or 6-carboxyfluorescein, under conditions for forming amide linkages, to yield a tracer. The tracer forms a complex with antiserum specific to digitoxin, in proportion to the relative concentration of the tracer with respect to the concentration of the digitoxin present in the sample. It has been found that excellent results are obtained when such a reagent is used for measuring concentrations of digitoxin.
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