RECOMBINANT DNA METHOD FOR PRODUCTION OF POLYPEPTIDES IN VERTEBRATE CELL CULTURE;PRODUCTION OF HEPATITIS B SURFACE ANTIGEN

摘要

A vertebrate cell culture is enabled to produce a desired polypeptide by transformation with a vector whose DNA comprises (a) means for replication in the host; (b) a promoter; and (c) a gene encoding the polypeptide, under the control (b). E.g. a recombinant genome was constructed comprising DNA from (a) the SV40 virus with excision of the VP-1 gene (-hence it is noninfective); (b) the HBsAg gene; and (c) pBR322 (rendering it TETR, and allowing cloning in E. coli). Cells of the CV-1 line of monkey kidney fibroblasts were co-infected therewith and incubated. Lysis occurred, liberating HBsAg which is required for hepatitis vaccine. Non-lytic vectors for expressing HBsAg were also prepared.