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IMMOBILIZED METAL ION AFFINITY CHROMATOGRAPHY PROTEIN PURIFICATION
IMMOBILIZED METAL ION AFFINITY CHROMATOGRAPHY PROTEIN PURIFICATION
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机译:固定化金属离子亲和层析蛋白的纯化
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摘要
This invention describes a process for separating a biologically active polypeptide or protein in the form of its precursor from a mixture containing said precursor and impurities, which comprises contacting said precursor with a resin containing immobilized metal ions, said precursor comprising the biologically active polypeptide or protein covalently linked directly or indirectly to an immobilized metal ion chelating peptide, binding said precursor to said resin, and selectively eluting said precursor from said resin. Such precursor compounds are also described.
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