Glutathione peroxidase, process for production thereof, method and composition for the quantitative determination of lipid peroxide

摘要

Glutathione peroxidase is produced by fermentation of a microorganism of the genus Mucor. The peroxidase is selenium free and may be used for the determination of lipid peroxide by oxidation of a reduced glutathione in the presence of the glutathione peroxidase, blocking the unreacted reduced glutathione, and determining the oxidised glutathione. Also disclosed is a reagent for the determination of lipid peroxide comprising the glutathione peroxidase, reduced glutathione, an SH blocking agent, alkali and a fluorescent agent.