Recombinant ricin fragments, vectors and transformed hosts expressing the same, the modification of DNA sequences, and isolation of mRNA

摘要

Recombinant vectors and methods for constructing ricin B are disclosed. The coding sequence for ricin B was cloned, disposed in suitable expression vectors and produced free of components normally accompanying this peptide. In addition, a novel means of reconstructing missing portions of coding sequence, and certain improvements in messenger RNA purification are disclosed.