Methods for separating and purifying young chestnuts from garlic scales

摘要

1) wash-removing the film scale from scabbard fish; 2) adjusting the scale-containing waste water to PH 5.0-6.0 with 0.1N H2SO4; 3) coagulate-separating org. mixtures contg. the scale with addition of an org. coagulant; 4) dissolveeliminating protein from the separated waste in 5-10% saline water of 10 times the waste volume or in a buffer soln (NaH2PO4 0.53g + Na2HPO4 2.20g + water 1 liter) of 5-10 time the waste volume; 5) elute-eliminating fat with the use of solvent mixture (acetone or MeOH: CHCl3 = 1:3). The product is used for cosmetics and for glitter of accessories.