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Process for the preparation of a liquid nutrient medium for the cultivation of microorganisms and cultures of cells of macroorganisms

机译:制备用于培养微生物和大微生物细胞培养物的液体营养培养基的方法

摘要

A process for the preparation of solid nutrient medium for the cultivation of microorganisms and cultures of cells of macroorganisms is proposed and entails the copolymerisation of acrylamide and N,N'-methylene-bis-acrylamide in a ratio by mass of 15.0-20.0:0.019-0.132, and the polyacrylamide gel produced after washing being kept in a physiological solution until the mass has increased by 2.8-4.5 times, with a subsequent treatment with liquid nutrient medium, or the polyacrylamide gel obtained after washing being exposed to swelling in a liquid nutrient medium until the mass has increased by 2.5-3.5 times.
机译:提出了一种用于培养微生物和大细胞的细胞的固体营养培养基的制备方法,该方法需要使丙烯酰胺与N,N'-亚甲基-双丙烯酰胺按质量比为15.0-20.0:0.019进行共聚。 -0.132,并且将洗涤后产生的聚丙烯酰胺凝胶保持在生理溶液中直至质量增加2.8-4.5倍,随后用液体营养培养基处理,或者将洗涤后获得的聚丙烯酰胺凝胶暴露于液体中溶胀营养物质,直到质量增加了2.5-3.5倍。

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