Hybrid cell lines producing monoclonal antibodies directed against neurotransmitter degrading enzymes

摘要

A continuous hybrid cell line which produces monoclonal antibody directed against the neurotransmitter degrading enzyme, monoamine oxidase B (MAO B), has been developed. The hybrid cell line was established by fusing MAO B primed, differentiated lymphoid cells with myeloma cells. Resulting fused cells were isolated, cloned and characterized as to antibody specificity against antigenic determinants of MAO B. Monoclonal antibody having specificity for MAO B and no cross reactivity with MAO A was selected and implemented in a radioimmunoassay technique for the selective measurement of MAO B concentration independent of its catalytic activity.