Reagent for measuring direct bilirubin by enzymatic method and method for measurement thereof

摘要

Reagent for the measurement of direct bilirubin comprising a buffer solution having a pH range of 3.5 to 4.5 which contains bilirubin oxidase, preferably in the form of a test kit consisting essentially of (i) a buffer solution having a pH range of 3.5 to 4.5, (ii-a) a lyophilized bilirubin oxidase, (ii-b) a buffer solution for dissolving the lyophilized bilirubin oxidase, and (iii) a standard serum containing a prescribed amount of bilirubin, and a method for measurement of direct bilirubin by using the reagent. The reagent and method are useful for diagnosis of various diseases, for example, various hepatic diseases (e.g. jaundice) and cholepathia.