antigenisk peptide isolated from 28 kd protein cream.mansoni and procedure for its isolation, monoclonal antibodies that recognize at least one epitope of the peptide itself and its application to the synthesis of antibodies

摘要

Peptide fragments isolated from the S. mansoni 28 KD protein by controlled proteolysis. Process for the isolation of a peptide bearing at least one epitope, by proteolysis of the S. mansoni 28 KD protein with V8 protease, the proteolysis then being stopped in order to collect, after heating to 100 DEG C, a peptide whose antigenic activity is verified using the antibodies of a polyclonal serum or monoclonal antibodies. Use in the preparation of vaccinating compositions against S. mansoni.