PROCESS FOR THE PREPARATION OF HUMAN CHORIONIC GONADOTROPIN (HCG) FROM PREGNANT HUMAN URINE

摘要

A process for the isolation of human chorionic gonadotropin (HCG) from the urine of pregnant women comprises concentrating the urine by lyophylisation, adding to the concentrated urine glycerol, acetone and sodium chloride, vigorously stirring the mixture, separating the upper layer, extracting the lower layer with chilled acetone, adding the extract to the separated upper layer, lyophylising the mixture, dissolving in phosphate buffer and dialised.