POLIOVIRUS-NEUTRALIZING-MONOCLONAL ANTIBODIES AND HYBRIDOMAS SECRETING SAID ANTIBODIES

摘要

ABSTRACT OF THE DISCLOSUREPOLIOVIRUS NEUTRALIZING MONOCLONAL ANTIBODIES REACTINGWITH PEPTIDE STRUCTURES OF THE POLIOVIRUS RESPONSIBLEFOR THE INDUCTION OF AN ANTIPOLIOMYELITIC IMMUNITY;HYBRIDOMAS SECRETING SUCH ANTIBODIES AND PROCESS FORTHEIR PRODUCTION.The invention relates to poliovirusneutralizing monoclonal antibodies capable of reactingwith peptide structures of the poliovirus responsible forthe induction of antipoliomyelitic immunity as well as tothe corresponding secreting hybridomas. A process formaking such hybridomas comprises fusing spleen cells ofan animal previously immunized against polioviruswith "C" antigenicity and myeloma cells, selecting thoseof the hybridomas secreting antibodies active both againstpolioviruses with "C" and "D" antigenicities and againstthe capsid VP1-structural peptide.