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Immunohistochemical assay for detection of a tumor-associated marker (p53)
Immunohistochemical assay for detection of a tumor-associated marker (p53)
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机译:免疫组织化学法检测肿瘤相关标志物(p53)
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摘要
This invention is an immunohistochemical p53 assay method in which human tissue sections or cells are fixed on a glass slide and incubated with anti-p53 antibodies which react with the p53 antigen in the specimen. The p53 antigen-antibody complex is then localized by addition of anit-lg which is either labeled directly or indirectly with an enzyme. Next, a chromogen substrate solution for the enzyme is added to the slide and color is developed. The slide is then examined under a microscope for specifically stained cells which indicate the presence of p53 antigen.
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