Nif Promoter of fast-growing rhizobium japonicum

摘要

The promoter of the nitH gene of the fast-growing Rhizobium japonicum strain USDA 191, has been cloned. Over 4.2 kilobase pairs (kbp) of DNA were sequenced. Sequences encoding nitH and the 5'-end of nitD were identified, as were sequences involved in promoting operon transcription and a nifH ribosome binding site. Use of the nitH promoter to drive transcription in Rhizobium of heterologous structural genes is taught. Useful sequences and plasmids are also disclosed.