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Insolubilized invertase enzyme with a high specific activity, manufacturing process therefor and process using this enzyme

机译:具有高比活性的不溶性转化酶,其制备方法和使用该酶的方法

摘要

The invention, which relates to enzymes, concerns especially an insolubilised invertase attached by a covalent bond to a solid support, characterised in that the invertase is attached by covalency to glutaraldehyde which is bonded by covalency to a difunctional silane, itself fixed on porous silica particles having a particle size in the ranges from 150 to 250 mu m and a pore diameter within the range from 10 to 30 nm, this insolubilised invertase having a specific activity of at least 6000 units per gram of insolubilised enzyme. Use for the hydrolysis of sucrose in sweet glucose and fructose syrups.
机译:本发明涉及酶,尤其涉及通过共价键结合到固体支持物上的不溶性转化酶,其特征在于所述转化酶通过共价结合到戊二醛,戊二醛通过共价结合到双官能硅烷上,所述双官能硅烷本身固定在多孔二氧化硅颗粒上该不溶性转化酶具有150至250μm的粒径和10至30nm的孔径,其比活性为每克不溶化酶至少6000单位。用于水解甜葡萄糖和果糖糖浆中的蔗糖。

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