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radioimmunoassay for thyroid hormone and reagent for its implementation
radioimmunoassay for thyroid hormone and reagent for its implementation
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机译:甲状腺激素放射免疫测定及其实施试剂
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摘要
The method of this invention is characterized by the use of hydrolyzed cross-linked polyacrylamide particles to which have been bonded, by means of covalent bonds, antibiodies against the thyroid hormone to be determined. The particles selected are of a size which forms a stable hydrophyllic suspension. In the preferred embodiment a measured quantity of unextracted human serum is mixed together with a blocking agent in an amount sufficient to displace the thyroid hormone to be measured from thyroxine binding globulin (TBG), a radioactively labeled thyroid hormone of the type to be measured, and the antibody- polyacrylamide complex. The hormone to be measured is displaced by the blocking agent followed by the competitive binding of the labeled and unlabeled hormone to the antibody- polyacrylamide particles. The particles are readily separated from the sample liquid and the radioactivity of the particle material and/or in the liquid is determined. Particles containing Alcian yellow or blue dye facilitate practice of the method.
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