The in vitro culture of fungi from mycorrhizae has vesicles and arbuscules

摘要

1. A process for preparing in vitro in an axenic condition mycelium and/or mycorrhizae of fungi of the type Endogonaceae, especially of the genus Glomus, which process consists in removing vesicular-arbuscular mycorrhizae, on fungical structures originating from intra roots, cleaning them and sterilizing them, in axenic condition, that is to say sterilizing the mycorrhizae roots without destroying the vesicular-arbuscular mycorrhizae in the roots, and then placing the mycorrhizae in culture on a sterile agar nutrient medium provided with mineral elements, vitamins, especially of the B group, and sugar and to recover the mycorrhizae and/or mycelium after growth.