PROCESS OF CULTIVATION OF BABESIA CANIS, APPLICATION TO THE PREPARATION OF ANTIGENS AND VACCINES, AND ANTIGENS AND VACCINES AGAINST PIROPLASMOSIS

摘要

ERYTHROCYTES INFECTED BY THE BABESIA CANIS PARASITE ARE INCUBATED IN THE CULTURE ENVIRONMENT SUITABLE FOR THE SURVIVAL OF ERYTHROCYTES AND THE MULTIPLICATION OF PARASITES, IN THE PRESENCE OF HOMOLOGATED SERUM, IN A SENSITIVELY STATIC STATE, IN A NORMAL TO TERMAL TEMPERATURE ATMOSPHERE, HIGH FROM 34 TO 38C, THEN LOW TEMPERATURE, ESPECIALLY BETWEEN 0 AND 10C, THIS CYCLE CAN BE REPRODUCED. WE COLLECT THE SURNAGEANT THAT WE CENTRIFUGES AND THAT WE FILTER. A DOG PIROPLASMOSIS VACCINE IS PREPARED FROM THIS SURNAGER.