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METHOD OF OBTAINING ACTIVE PROTEIN FROM MATTER IN BIOLOGICALLY INACTIVE STATE
METHOD OF OBTAINING ACTIVE PROTEIN FROM MATTER IN BIOLOGICALLY INACTIVE STATE
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机译:在生物非活性状态下从物质中获得活性蛋白的方法
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摘要
There is described a process for preparing a biologically active spatial form of a protein from a biologically inactive spatial form, which is characterised in that the protein is dissolved, with the addition of a denaturating agent, and thus converted into the "random coil" form and the solution is allowed to pass through a material which has molecular sieve properties and which contains a liquid medium in which the protein can assume a biologically active spatial form, and this material with molecular sieve properties being selected such that the molecules of the denaturating agent can penetrate, but the protein molecules cannot. Centrifugation allows removal of the medium in the "external volume" of the molecular sieve and acceleration of the solution passing through the molecular sieve.
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