METHOD FOR ANALYZING MEDICINAL COMPONENTS OF GINSENG

摘要

PURPOSE:To analyze ginseside with high sensitivity without receiving the effect of impure components, by converting ginseside to sugar by hydrolysis using and acid and detecting sugar by a fluorescent method. CONSTITUTION:One end of a separation column 1 is connected to a mobilie phase tank 3 through a specimen injection port 2 and the other end thereof is connected to an acid liquid tank 4 for hydrolysis and further connected to a phosphorating reagent liquid tank 6 through a mixing coil 5. A fluorescence detector 8 is connected to the tank 6 on the outflow side thereof through a cooling tank 7. Acetonitrile and water are received in the tank 3 and an aqueous perchloric acid solution is received in the tank 4 and an aqueous L-alginic acid solution is received in the tank 6 to operate an apparatus. When a specimen is injected, ginseside of a specimen component is separated as a fixed phase and subsequently receives hydrolysis by perchloric acid to be converted to sugar. This sugar is phosphorated by the phosphorating reagent to flow in the detector 8 and emits the fluorescence proportional to concn. Since the other sugar component in the specimen is separated from the ginseside component by the column 1, said sugar does not obstruct analysis.