High level expression in E. Coli of soluble, human mature interleukin-1beta and derivatives with modified biological activity

摘要

Plasmid pUC8 and DNA coding for hIL-1 beta are used to construct hybrid plasmids capable of high level expression in E. coli of soluble proteins, including mature hIL-1 beta and derivatives of mature hIL-1 beta having amino acid substitutions and insertions at one or all of position 1 to 4 at the amino terminus. Derivatives of hIL-1 beta with alterations at the N-terminus have been produced which have either enhanced or decreased bioactivity compared to native monocyte derived hIL-1 beta .