首页> 外国专利> PLASMID VECTORS, PLASMIDES WITH GENES FOR ALKALINE PHOSPHATASES AND BACTERIA THAT CONTAIN THE LATTER, THEIR PRODUCTION AND USE.

PLASMID VECTORS, PLASMIDES WITH GENES FOR ALKALINE PHOSPHATASES AND BACTERIA THAT CONTAIN THE LATTER, THEIR PRODUCTION AND USE.

机译:质粒矢量,含有基因的质粒,用于含磷的碱性磷酸酶和细菌及其细菌,其生产和使用。

摘要

PhoA mutant E. coli SB44, prepared by mutation of E. coli HB101 with an N-nitroso compound, can be used to identify and isolate recombinant plasmids into which a phoA gene has been incorporated. These plasmids can be used to transform bacteria which can be cloned and incubated to provide alkaline phosphatase in high yield. Moreover, these plasmid vectors can be modified in various ways so that the N-terminal amino acid sequence of phoA is followed in reading phase by the DNA coding for some other protein. In turn, these new plasmids can be used to transform bacteria which can be cloned and incubated to produce fusion proteins comprising the desired other protein in high yield and outside of the cell membrane in the periplasmatic space.
机译:通过用N-亚硝基化合物使大肠杆菌HB101突变而制备的PhoA突变大肠杆菌SB44,可用于鉴定和分离已结合有phoA基因的重组质粒。这些质粒可用于转化细菌,可对其进行克隆和孵育以高产率提供碱性磷酸酶。而且,可以以各种方式修饰这些质粒载体,使得在阅读阶段,phoA的N-末端氨基酸序列后面跟随着编码其他蛋白质的DNA。继而,这些新质粒可用于转化细菌,所述细菌可被克隆和孵育以高产量并在周质空间的细胞膜外部产生包含所需其他蛋白质的融合蛋白。

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