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Assay for varicella-zoster virus (VZV) antibodies.
Assay for varicella-zoster virus (VZV) antibodies.
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机译:水痘带状疱疹病毒(VZV)抗体测定。
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摘要
Antibodies in mammalian biological fluids to each of the three major VZV glycoprotein groups, gA, gB and gC, can be detected, for example, by isolating each viral glycoprotein from VZV-invected cells in vitro, adhering these to a plastic surface and contacting with a biological fluid containing the suspected antibodies. Subsequent treatment with a conjugate consisting of, for example, an antibody-enzyme complex (with specificity for either one, two or all three glycoprotein groups), followed by contact with an enzyme substrate and subsequent determination of the quantity of reacted enzyme substrate, i.e., the ELISA assay, provides a relative measure of the antibody(ies) present directed against VZV glycoproteins.
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