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High level expression in E. coli of soluble mature hIL-1beta and derivatives with altered biological activity
High level expression in E. coli of soluble mature hIL-1beta and derivatives with altered biological activity
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机译:可溶性成熟hIL-1beta及其衍生物在大肠杆菌中的高水平表达及其生物学活性的改变
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摘要
Plasmid pUC8 and DNA coding for hIL-1β are used to construct hybrid plasmids capable of high level expression in E. coli of soluble proteins, including mature hIL-1β and derivatives of mature hIL-1β having amino acid substitutions and insertions at one or all of position 1 to 4 at the amino terminus. Derivatives of hIL-1β with alterations at the N-terminus have been produced which have either enhanced or decreased bioactivity compared to native monocyte derived hIL-1β .
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