Brief description of the immunological determination of the hormones thyroid t3 and / or t4 using thyroglobulin

摘要

The invention has for its object a process for the immunological determination of the hormones thyroid t and or t presented in free form in a sample. / p & & p & this process consists in placing in competition t and or t is determined with thyroglobulin to the sites of antibody - t and or anti - t presents in a quantity limited and in then determining the quantity of thyroglobulin fixed to the antibody - t and or anti - t, i.e. the quantity of thyroglobulin not fixed to the antibody - t and or anti - t. usually thyroglobulin is immobilized on a solid support. The antibody - t or anti - t may be marks directly, for example, by an element radioactive, fluorescent, luminescent or an enzyme, or else be reveles so indirectly by other antibodies marks. / p & & p & this process can be used for determining the t and or the total of a biological sample is provided to add to the sample an inhibitor in order to release the element t and or the t linked a of the carrier protein.