DEFECTIVE RECOMBINANT RETROVIRUS, METHOD FOR MANUFACTURING THE SAME AND ITS APPLICATIONS, IN PARTICULAR FOR THE STUDY OF THE INTERACTION OF WILD RETROVIRUSES WITH CELLULAR LINES INFECTIVE THEREWITH

摘要

The invention relates to means for studying the interactions of a retrovirus defined with cell lines that can be infected by this retrovirus. IT INVOLVES A DEFECTIVE RECOMBINANT RETROVIRUS DERIVED FROM THE RETROVIRUS IN QUESTION, CONTAINING THE PARTIES ACTING IN "CIS" OF THIS RETROVIRUS WHICH NORMALLY ENSURES ITS PACKAGING, ITS RESERVATION-TRANSCRIPTION IN A DNA MOLECULE AND ITS INTEGRATION IN THE GENOME OF THE CELL-HOST THIS RECOMBINANT RETROVIRUS IS CHARACTERIZED BY PARTIAL OR TOTAL SUBSTITUTION OF THE SEQUENCES ENCODING ITS VIRAL PROTEINS BY A GENE SEQUENCE ENCODING ITSELF FOR A PROTEIN ACTING AS A MARKER OF INFECTED CELLS. DETECTION OF THE MARKER IN CELLS OF SENSITIVITY TO INFECTION IS STUDYED OF THEIR CAPACITY TO BE INFECTED BY WILD RETROVIRUS. / P