REGENERATION OF SPHEROPLAST OF BACTERIUM BELONGING TO GENUS ACETOBACTER

摘要

PURPOSE:To regenerate the titled spheroplast for breeding a bacterium for producing vinegar in high efficiency, by cultivating spheroplast of a bacterium belonging to the genus Acetobacter in a solid medium containing a disaccharide, monosaccharide or sugaralcohol. CONSTITUTION:A bacterium [e.g. Acetobacter aceti No.1,023 (FERM P-7122)] belonging to the genus Acetobacter is treated with a cell wall digesting enzyme (e.g. glair lysozyme) to prepare spheroplast, which is cultivated in a solid medium (e.g. agar medium) containing one or more of a disaccharide such as sucrose, maltose, isomaltose or lactose, monosaccharide such as glucose, mannose or xylose and a sugaralcohol such as sorbitol, mannitol or xylitol to regenerate spheroplast of a bacterium belonging to the genus Acetobacter.