INDOPHENYL-BETA-D-GLUCOSIDE DERIVATIVE, PRODUCTION THEREOF AND UTILIZATION THEREOF TO REAGENT FOR MEASURING BETA-GLUCOSIDASE ACTIVITY

摘要

NEW MATERIAL:A compound expressed by formula I (R is H or acyl; X1-X6 are H, halogen, nitro, cyano, azido, acyl, sulfonic acid, nitroso, sulfonyl, sulfoxyl, thiocyano, isothiocyano, isonitrile, imino, azo, diazo, alkyl, allyl or aryl or X3 and X4 and X5 and X6 may be each together linked to form condensed aromatic ring). EXAMPLE:Phenolindo-3'-chlorophenyl-beta-D-glucoside. USE:A reagent for measuring beta-glucosidase activity. PREPARATION:A 4-aminophenyl-beta-D-glucoside derivative (e.g. 2-chloro-4- aminophenyl-beta-D-glucoside) expressed by formula II is reacted with a quinone derivative expressed by formula III to provide the aimed compound expressed by formula I.