CULTURE MEDIUM OF PROTOPLAST DERIVED FROM HIGHER PLANT AND CULTURE THEREOF

摘要

PURPOSE:To raise organism activity of protoplast, by adding reduction type glutathione and/or glutathione peroxide to a medium to prepare a culture medium for higher plant derived protoplast. CONSTITUTION:Reduction type glutathione and/or glutathione peroxide is added to a medium such as Murashige and Skoog medium, Nagata and Takebe medium or Nitsch and Nitsch medium to prepare a culture medium for higher plant derived protoplast. Reduction type glutathione of any origin may be used as the reduction type glutathione and the concentration thereof added is preferably about 0.1-10mM. Glutathione peroxide derived from baker's yeast is preferable as the glutathione peroxide and the amount thereof added to the medium is preferably about 1-100 units. Phospholipase A2 may be added to the medium.