RECOMBINANT DNA, ITS PRODUCTION, EUCARYOTIC OR PROCARYOTIC METABOLITE AND METHOD FOR OBTAINING PROTEIN PRODUCED BY GENE ENGINEERING TECHNIQUE

摘要

A recombinant DNA contains DNA sequences coding for the N-terminal membrane-penetrating domain of the E protein of phage phi X174, and DNA sequences coding for the C-terminal membrane-penetrating domain of the L protein of phage MS2, and the DNA sequences of the two phages are connected by a DNA sequence coding for a hydrophilic flexible amino-acid sequence. It encodes a lytic enzyme. Incorporated in a vector, it is suitable for obtaining eukaryotic or prokaryotic metabolic products or genetically engineered proteins by partial or complete lysis of the cell wall of the cells or microorganisms used for the expression by expression of the lytic protein encoded by the recombinant DNA. …IMAGE…