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METHOD AND APPARATUS FOR EXTRACTING INTERNAL SECRETION ISLAND FROM DONOR ORGAN AND USE THEREOF FOR TREATING METABOLIC DISEASE
METHOD AND APPARATUS FOR EXTRACTING INTERNAL SECRETION ISLAND FROM DONOR ORGAN AND USE THEREOF FOR TREATING METABOLIC DISEASE
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机译:从施主器官中提取内分泌岛并用于治疗代谢性疾病的方法和装置
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摘要
A process and an apparatus for isolating endocrine islets from a donor organ, for example from a pancreas, and the use of the isolated islets are described. In this process, the donor organ is artificially perfused with a mixed blood substance during a perfusion phase and then immersed in collagenase fluid until the fibre material separates during a dissolution or digestion phase until a homogeneous fragment suspension is formed. The fragment suspension is centrifuged to isolate a sediment from exocrine cell material and endocrine cells, so that in an islet isolation phase the dead endocrine cell contents can be separated from the live endocrine islets. The atraumatically isolated islets can be transplanted or injected for the resumption of the normal metabolic function of an organ. IMAGE
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