RAT NADH-CYTOCHROME B5 REDUCTASE GENOMIC DNA

摘要

PURPOSE:To obtain a rat reduced form nicotinamide adenine dinucleotide (NADH)-cytochrome b5 reductase (b5 R) genomic DNA, by cultivating a bacterium containing a plasmid.vector, containing a nucleotide sequence within the 5'-region and exon region having a specific sequence and integrated therein. CONSTITUTION:A bacterium modified so as to contain a plasmid.vector containing a nucleotide sequence in which a nucleotide within the 5'-region and exon region has a specific sequence or a functional equivalent of the above- mentioned nucleotide sequence is prepared. This bacterium is then cultivated to provide a rat NADH-cytochrome b5 R genomic DNA clone usable for synthesizing b5 R by using a suitable cell, e.g. animal cell, as a host.