COMPOSITIONS CONTAINING A BORATE BUFFER,DEVICE AND METHOD FOR ENZYMATIC SEMIQUANTITATIVE DETERMINATION OF HIGH RANGE GLUCOSE IN BIOLOGICAL AQUEOUS FLUIDS

摘要

A test composition comprising glucose oxidase, a peroxidatively active component, a chromogenic indicator system capable of providing a detectable response and a borate buffer capable of providing an initial pH above about pH 7 is particularly useful for the semiquantitative determination of high range glucose, (i.e. glucose concentrations of 1, 000 mg/dL to 10,000 mg/dL). A preferred indicator system is a water soluble iodide salt and poly(vinylpyrrolidone). The use of a borate buffer capable of providing an initial pH above about pH 7 permits greatly improved resolution for the semiquantitative determination of high range glucose when the test composition is incorported onto a carrier matrix to prepare a solid state unitary test device.