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TRANSFORMATION OF YARROWIA LIPOLYTICA WITH FURTHER CHROMOSOMAL DNA AND A DECTABLE GENETIC MARKER: VECTORS, PLASMIDS, AND TRANSFORMANTS AND PROCESSES
TRANSFORMATION OF YARROWIA LIPOLYTICA WITH FURTHER CHROMOSOMAL DNA AND A DECTABLE GENETIC MARKER: VECTORS, PLASMIDS, AND TRANSFORMANTS AND PROCESSES
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机译:用进一步的染色体DNA和可降解的遗传标记转化羊脂多糖的脂溶性:矢量,质粒,转化子和过程
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摘要
Process for transformation of Yarrowia lipolytica, vectors useful therefor comprising DNA of a microbial vector and chromosomal DNA of Y. lipolytica and transformants comprising said vectors in E coli and Y lipolytica, and integrative shuttle vectors for Escherichia-Yarrowia transgeneric cloning. Said vectors or subclones thereof enable creation of Y. lipolytica cloning vectors into which specific or random segments of DNA can be inserted and the resulting vectors used to transform a suitable host microbe, especially Y. lipolytica, to improve the fermentation characteristics thereof and hence their industrial utilization. The methodology described permits the cloning of genes from a gene library of Y. lipolytica by complenentation with an integrating vector. Specifically claimed are the plasmid vectors pLD25, pLD28, pLD40 and pLD55, Y. lipolytica transformants containing pLD25 and pLD55, and an E. coli transformant containing pLD25.
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