IN SITU DETECTION OF HUMAN T-CELL LEUKEMIA VIRUS TYPE III

摘要

ABSTRACTIn situ hybridization method for the detectionof viral RNA or DNA, involving the hybridization of atissue sample with an 35S-labeled HTLV-III RNA probe.The probe detects HTLV-III in primary, uncultured tissuesamples, is highly sensitive, and is relatively fastacting. The RNA probe may be synthesized from clonedHTLV-III DNA inserted into a suitable transcriptionvector.