Process for the detection of the mutagenic potency of substances capable of inducing cellular DNA damage, using the production of an SOS response

摘要

A method for the detection of the mutagen effect of a substance or composition by producing culture of a micro-organism harboring a recombinant of a sfi gene and of a gene coding for a dosable enzyme in the presence of said substance or composition and measuring the activity of the dosable enzyme induced under the control of the sfi gene. When the latter is activated due to the mutagen character of said substance or composition, measuring the activity of a distinct enzyme synthesized by the microorganism and coded by a gene not involved in the activation process of the sfi gene and measuring the variation of the ratio of the activity of the abovesaid dosable enzyme to the activity of said distinct enzyme. Then comparing the variation that of the ratio of the activities of the same enzyme in a culture of the same microorganism, but in the absence of the mutagen substance.