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A method for the expression of recombinant genes under stimulation from an inducively expressed activator protein

机译:在诱导表达的激活蛋白刺激下表达重组基因的方法

摘要

A DNA system for the stimulated expression of genes of interest. Said genes are placed under the control of an activator stimulated promoter and the gene coding for this activator is driven by an inducible promoter. The hybrid DNA constructions are incorporated in suitable host cells which, after multiplication and optional gene amplification, are stressed, whereby the activator produced continues to bring about expression of said genes of interest for a long period of time after the inducible promoter has retur­ned to rest.
机译:用于刺激目的基因表达的DNA系统。所述基因置于活化剂刺激的启动子的控制下,编码该活化剂的基因由诱导型启动子驱动。将杂交DNA构建体掺入合适的宿主细胞中,所述宿主细胞在繁殖和任选的基因扩增后受到压力,由此产生的激活剂在诱导型启动子恢复静止后的很长一段时间内继续引起所述目的基因的表达。 。

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