DNA PROBE/ANTIBODY BASED ASSAYS AND INTERMEDIATES USEFUL IN THE SYNTHESIS OF CLEAVABLE NUCLEIC ACIDS FOR USE IN SUCH ASSAYS

摘要

The assay uses a labelled solid phase component (s.p.c.) which carries a member of a specific binding pair (s.b.p.) and a member of a signal producing system. A second larger and magnetic s.p.c. may also be used which carries an s.b.p. member capable of binding to the complementary s.b.p. member simultaneously with the labelled s.p.c. so as to facilitate separation of bound from unbound components. A further s.p.c. may be used in a pre-treatment to remove non-specific binding elements from the complementary s.b.p. member. Where the target s.b.p. member is a nucleic acid, it may be captured by a thionucleotide probe immobilized on a s.p.c., and subsequently released from the s.p.c. by cleavage of the P-S bonds in the probe. Suitable oligonucleotide probes containing P-S bonds are prepared by solid phase synthesis using a thiophosphoramidite intermediate. Also disclosed are compounds of formula IMAGE where B is a DNA or RNA base and -P-Y is preferably IMAGE