Serum pretreatment in digoxin immunoassay

摘要

A method is disclosed for preparing a sample suspected of containing digoxin for determination of digoxin in the sample during an assay. The method comprises contacting the sample with &bgr;-cyclodextrin in an amount and under conditions sufficient to allow a substantial portion of digoxin in the sample to bind to the &bgr;-cyclodextrin. The &bgr;- cyclodextrin with bound digoxin is separated from at least one other component of the medium. Next, the digoxin is released from the &bgr;- cyclodextrin to provide a sample containing digoxin which may be analyzed by any of a number of assay techniques.