Cloning and expression of the 47-kilodalton antigen of treponema pallidum

摘要

Monoclonal antibodies directed against the 47 kDa major outer membrane surface immunogen of virulent Treponema pallidum were used to select E. coli recombinant clones expressing the 47 kDa immunogen. The phenotype of the clones was dependent on the presence of recombinant plasmid in the host cell. Southern hybridization revealed thatP PThe United States government may have rights in the substance of this patent because of developmental work supported by the U.S. Department of Health and Human Services in the form of research grants 1- R01-AI-16692 and 1- R01-AI-17366 from NIH-NIAID.