METHOD FOR FORMING AND PROLIFERATING CROWN OF PLANT OF GENUS ASPARAGUS AND PRODUCTION OF SEEDLING

摘要

PURPOSE:To enable the production of the seedling of the subject plant in high efficiency by culturing a tissue or a callus of the plant in a medium containing 10-7-10-4M of cytokinin, culturing the formed crown and acclimatizing the grown plant body. CONSTITUTION:Tissue (e.g. embryo or shoot apex) of a plant of genus Asparagus or a callus produced by the culture of the tissue is subjected to tissue culture in a medium produced by adding a carbon source (e.g. sucrose), 10-7-10-4M of cytokinin, a plant hormone, vitamins, etc., to a medium such as Murashige- Skoog medium to form a crown. If necessary. the crown is subjected to thickening growth and then cultured and rooted in a medium produced by adding a carbon source, plant hormones, amino acids and 10-8-10-5M of auxin to obtain a plant body of a proper size. The grown plant body is acclimatized to obtain a seedling. Plant body and seedling can be produced in large quantities and high efficiency by this process.