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GLYCEROALDEHYDE TRIPHOSPHATE DEHYDROGENASE GENE PROMOTER AND USE THEREOF
GLYCEROALDEHYDE TRIPHOSPHATE DEHYDROGENASE GENE PROMOTER AND USE THEREOF
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机译:甘油醛酸三磷酸脱氢酶基因启动子及其用途
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摘要
PURPOSE:To improve the productivity of cephalosporins by integrating a glyceroaldehyde triphosphate dehydrogenase gene(GLDG) originated from Acremonium chrysogenum(ACG) into a plasmid. CONSTITUTION:(A) A DNA fragment having e.g. the base sequence of formula containing the GLDG promoter active part of ACG is cut from the chromosome DNA of ATCC 11550 strain, etc., of ACG. The GLDG promoter of ACG is integrated to obtain a plasmid (B). A plasmid (C) is produced by integrating a GLDG translation initiation site at the downstream of the GLDG promoter site of ACG. A GLDG structure gene is integrated to the downstream of the promoter or translation initiation site of the components B and C to obtain (D) a plasmid such as pGH2. A transformant capable of synthesizing cephalosporins and obtained by the transformation with the component D is aerobically cultured in a medium containing glucose, etc., at pH5-9 and 20-30 deg.C for 4-14 days to obtain cephalosporins.
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