NOVEL HEPARAN SULFATE DEGRADATION ENZYME AND MICROORGANISM PRODUCING SAME AND METHOD FOR PRODUCING SAME ENZYME

摘要

NEW MATERIAL:Heparitinase I, IV and V. action: a lyase acting on the glucosaminide linkage in heparin or heparan sulfate and forming double bond between the 4-site and 5-site carbons in glucuronic acid or iduronic acid as a scission part; substrate specificity: heparitinase I decomposes heparan sulfate to form non-sulfated product, N-acetyl glucosamine-6-sulfate, glucosamine-N- sulfate and small amount of glucosamine-N,6-disulfate, each unsaturated biose. USE:A molecular weight-reducing agent in preparing low-molecular weight heparin as an anti-thrombogenic agent, a reagent for structural analysis of heparan sulfate. PREPARATION:Bacteria classified as Flavobacterium [e.g., Hp206 strain (FERM No.10207)] capable of producing heparitinase I, IV and V is put to culture and the resultant enzyme is separated and collected from the culture solution or a liquid extract from the bacteria.